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目的观察硫酸化人参皂苷(sGS)抗新城疫病毒的活性。方法采用氯磺酸-吡啶法制备sGS,红外光谱仪对sGS结构进行分析,MTT法检测硫酸化修饰前后人参皂苷(GS)预先给药、接种病毒后给药、与病毒同时给药3种不同给药方式的抗新城疫病毒活性。结果硫酸化修饰后人参皂苷在1 144、807 cm?1有2个特征吸收峰,表明硫酸基已经和糖链结合成酯。预先给药或病毒接种后给药,加入一定质量浓度范围内的GS和sGS的CEF培养液的A570值均显著大于未给药的病毒组(P<0.05),且GS与sGS的抗病毒活性无显著差异(P>0.05);GS和sGS与病毒同时给药,两药在一定质量浓度范围内的A570值也均显著大于未给药的病毒组(P<0.05),且sGS的最大病毒抑制率显著高于GS。结论 GS经硫酸化修饰后可提高其抗新城疫病毒活性,主要表现为提高其直接杀灭病毒的作用。
Objective To observe the activity of sulfated ginsenosides (sGS) against Newcastle disease virus. Methods sGS was prepared by chlorosulfonic acid-pyridine method. The structure of sGS was analyzed by infrared spectroscopy. Ginsenosides (GS) before and after sulfation modification were assayed by MTT assay. After inoculation with virus, three different doses Anti-Newcastle disease virus activity. Results Sulfated modified ginsenosides showed two characteristic absorption peaks at 1 144 and 807 cm -1, indicating that the sulfate group has been combined with sugar chains to form esters. After pre-administration or post-virus inoculation, the A570 values of CEFs supplemented with GS and sGS in a certain concentration range were significantly higher than those of untreated virus (P <0.05), and the antiviral activity of GS and sGS (P> 0.05). GS and sGS were administered simultaneously with the virus. The A570 values of both drugs in a certain concentration range were also significantly higher than those in the untreated group (P <0.05), and the maximum sGS virus The inhibition rate was significantly higher than GS. Conclusion GS sulfated modified to improve its activity against Newcastle disease virus, mainly to improve its role directly kill the virus.