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目的：建立测定西替利嗪在人体血浆中浓度的反相离子对高效液相色谱法（ＲＰ－ＨＰＬＣ）。方法：以Ｎｏｖａ－ＰａｋＣ１８４μｍ色谱柱为分离柱，流动相为乙腈∶磷酸盐缓冲液（０．０２ｍｏｌ·Ｌ－１ＮａＨ２ＰＯ４）∶三乙胺（５０∶５０∶０．１５，ｐＨ３．１５，内含十二烷基硫酸钠（ＳＤＳ）０．００９５ｍｏｌ·Ｌ－１，普萘洛尔为内标，ＵＶ检测波长２２９ｎｍ。结果：方法线性范围１０～４００ｎｇ·ｍｌ－１（ｒ＝０．９９９８），平均回收率为９８．１％±０．８％，日内和日间精密度均小于３％。结论：应用本法研究西替利嗪片剂在正常人体内的药动学，具有简便、迅速、准确之特点
Objective: To establish a reversed-phase ion-pair high performance liquid chromatography (RP-HPLC) for the determination of cetirizine in human plasma. Methods: Nova-Pak C184μm column was used as the separation column. The mobile phase consisted of acetonitrile: phosphate buffer (0.02mol·L-1NaH2PO4): triethylamine (50:50:0.15, pH3.15, (SDS) 0.0095mol·L-1, propranolol as internal standard, UV detection wavelength 229nm.RESULTS: The linear range of the method was 10-400ng · ml-1 (r = 0.9998) The recoveries were 98.1% ± 0.8%, and the intra-day and inter-day precision were less than 3% .Conclusion: This method was applied to study the pharmacokinetics of cetirizine tablets in normal human body with simple, rapid, Accurate features