作者采用未培养的原始肿瘤组织进行细胞遗传学研究。切碎肿瘤组织使之变成细胞悬浮液。细胞暴露在秋水仙硷中90分钟,37℃,接着做低渗处理。用醋酸:甲醇[1:3]固定。根据巴黎会议的标准确定核型。本文研究了40个细胞中期分裂相,其中4个有46条染色体的正常核型,其余36个中期分裂相有51～76条染色体,大部分染色体数 The authors used uncultured primary tumor tissue for cytogenetic studies. Tumor tissue is shredded to become a cell suspension. Cells were exposed to colchicine for 90 minutes at 37 ° C followed by hypotonic treatment. Fixed with acetic acid: methanol [1: 3]. Karyotypes are based on the criteria of the Paris meeting. In this study, 40 cell metaphase cells were studied, of which 4 had 46 karyotypes of normal karyotypes, and the remaining 36 metaphases had 51-76 chromosomes, most of which