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本实验研究了氯丙烯亚急性染毒对大鼠肝、肾组织谷胱甘肽(GSH)、肝细胞溶质的谷胱甘肽-S-转移酶(GST)和肝微粒体细胞色素P-450的影响,以探索代谢途径与氯丙烯毒作用的关系。实验发现,氯丙烯1/4LD_(50)剂量染毒14天,引起肝肾GSH含量显著增加,GST和细胞色素P-450活性明显升高;剂量降至1/15LD_(50),连续染毒28天,仅见到GST活性下降和肾GSH含量增加。实验表明,细胞色素P-450和GST活性的诱导有利于氯丙烯在体内的代谢解毒,而不同剂量不同染毒时间所致不同酶活性的变化则可能是亚急性和慢性氯丙烯中毒产生不同毒作用的基础
In this study, we investigated the effects of sub-acute exposure to allyl chloride on glutathione (GSH), glutathione-S-transferase (GST) and hepatic microsomal cytochrome P-450 To explore the metabolic pathway and the relationship between the role of allyl chloride poisoning. The results showed that the GSH content in liver and kidney increased significantly and the activity of GST and cytochrome P-450 increased markedly after exposure to 1 / 4LD_ (50) of allyl chloride for 14 days. The dose was reduced to 1 / 15LD_ (50) At 28 days, only a decrease in GST activity and an increase in renal GSH content were seen. Experiments show that the induction of cytochrome P-450 and GST activity is conducive to the metabolism of allyl chloride in vivo detoxification, and different doses of different exposure time due to different enzyme activity changes may be subacute and chronic allyl chloride poisoning produce different toxic The basis of the role