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目的 检测口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)中血管生成抑制蛋白-1 (Vasohibin-1,VASH-1)和 LGR5的表达及两者的相关性,分析两者与OSCC临床病理特征的关系.方法 采用免疫组化EliVision法检测223例OSCC和80例正常口腔黏膜组织中VASH-1和LGR5的表达.结果 在OSCC组织中,VASH-1和LGR5的阳性率分别为59.6％、6％;正常口腔黏膜组中VASH-1和LGR5的阳性率分别为12.5％和13.8％,两组相比差异有统计学意义(P＜0.05). VASH-1和LGR5的阳性率与OSCC肿瘤大小、病理分级、淋巴结转移及TNM分期均呈显著正相关,差异有统计学意义(P＜0.05);与OSCC患者年龄、性别、肿瘤位置、吸烟及饮酒等无相关性(P＞0.05).Spearman等级相关分析发现,VASH-1与 LGR5表达呈正相关(rs =0.718,P＜0.001).结论 VASH-1和LGR5在OSCC中表达均增高,可能参与OSCC发生、发展,并可能在OSCC的浸润、转移和预后中发挥重要作用.“,”Purpose To detect the expression of vasohibin-1 (VASH-1) and LGR5 protein in oral squamous cell carcinoma(OSCC) and their clinical significances. Methods Expression of VASH-1 and LGR5 protein in 223 cases of OSCC tissues and 80 cases of adjacent normal oral mucosa tissues was examined by the immunohistochemicalEliVision method. Results The positive rate of VASH-1 and LGR5 protein in OSCC tissues and the control tissues was 59.6％, 55.6％ and 12.5％, 13.8％, respectively. There was a significant difference between the two groups (P＜ 0.05 ). The expression of VASH-1 and LGR5 protein was significantly related with grades of tumors, size of tumors, lymph node metastasis, and TNM stages (all P＜0.05). The positive expression of VASH-1 and LGR5 was not relevant to age, gender, location of tumor, smoking, and alcohol (all P＞ 0.05). Spearman correlation analysis showed that there was a positive association between the VASH-1 expression and LGR5 expression (rs =0.718, P＜0.001). Conclusion The expression of VASH-1 and LGR5 in OSCC is high and may promote the initiation and development of the cancer, which play an important role in the invasion, metastasis, and prognosis of OSCC.