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目的观察痰瘀同治方对阿尔茨海默病(AD)小鼠学习认知能力、脑组织氧化应激和神经炎性反应的影响。方法将100只雄性小鼠随机分为5组:对照组,模型组,痰瘀同治方高、中、低剂量组,每组20只。除对照组外,在其他各组小鼠侧脑室注射β淀粉样肽(Aβ)1-40,建立AD小鼠模型,连续14周给予相应药物灌胃。采用Morris水迷宫试验观察小鼠行为学的改变。麻醉后处死小鼠,采用比色法测定小鼠脑组织内丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)的含量及活性,免疫组织化学法测定脑内神经胶质元纤维酸性蛋白(GFAP)阳性细胞(星形胶质细胞)的表达。结果与模型组相比,痰瘀同治方可以明显改善小鼠学习认知能力(P<0.01),降低脑组织内MDA水平(P<0.05),增加SOD,GSH-PX和GSH含量及活性(P<0.05),降低星形胶质细胞表达数量(P<0.05)。结论痰瘀同治方通过抑制AD小鼠脑组织内氧化应激与神经炎性反应,明显改善AD小鼠的学习认知能力。
Objective To observe the effects of Phlegm and Blood Stasis Tongji prescription on cognitive abilities, oxidative stress and neuroinflammatory response in Alzheimer’s disease (AD) mice. Methods 100 male mice were randomly divided into 5 groups: control group, model group, phlegm and blood stasis with high, medium and low dose groups, 20 in each group. In addition to the control group, β-amyloid peptide (Aβ) 1-40 was injected into the lateral ventricle of other groups of mice to establish an AD mouse model, and the corresponding drugs were administered intragastrically for 14 weeks. Morris water maze test was used to observe the behavioral changes in mice. Mice were killed after anesthesia, and the content of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH) -PX), and the expression of glial fibrillary acidic protein (GFAP) positive cells (astrocytes) in brain were measured by immunohistochemistry. Results Compared with the model group, Phlegm and blood stasis syndrome could significantly improve the learning and cognitive abilities of mice (P <0.01), reduce the level of MDA in brain tissue (P <0.05), and increase the contents and activities of SOD, GSH-PX and GSH P <0.05), reduce the number of astrocytes (P <0.05). Conclusion Phlegm and blood stasis syndrome can significantly improve learning and cognitive abilities of AD mice by inhibiting oxidative stress and neuroinflammatory reaction in AD mice brain.