论文部分内容阅读
实验以盐生动物卤虫(Artemia)为材料,采用酚-氯仿法提取总DNA,以已报道的BADH基因的保守序列设计引物,通过PCR技术,从卤虫的基因组DNA中分离得到了1个BADH基因片段,经测序,得到一段868 bp的核苷酸序列,输入Gen-Bank进行BLAST同源性比较,发现所得片段与植物中的籽粒苋的BADH4基因中的一段序列有96%的同源性,此项研究为更深入地研究抗盐动物的BADH基因奠定了良好的基础.
In the experiment, Artemia was used as the material, total DNA was extracted by phenol-chloroform method, primers were designed based on the reported conserved sequence of BADH gene, and one PCR product was obtained from the genomic DNA of Artemia BADH gene fragment was sequenced to obtain a nucleotide sequence of 868 bp. GenBank was used for comparison of BLAST homology and the obtained fragment was found to be 96% homologous to a sequence in the BADH4 gene of the plant amaranth This study laid a good foundation for further study of BADH gene in salt-tolerant animals.