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目的观察chemerin对于TGF-β/smad通路的影响,探讨chemerin在糖尿病肾病发病机制中的作用。方法培养大鼠系膜细胞,分组干预:对照组加入DMDM培养液;高糖组中加入30 mmol/L葡萄糖;chemerin干预组加入0.5 mmol/L的chemerin蛋白;chemerin+高糖联合干预组中同时加入0.5 mmol/L的chemerin蛋白、30 mmol/L葡萄糖。72 h后留取细胞沉淀,realtime-PCR的方法检测各组细胞转化生长因子-β1(TGF-β1)、Smad2、Smad4表达水平的变化。结果与对照组相比,高糖组和chemerin组的TGF-β1、Smad2、Smad4的表达水平明显较高,而chemerin+高糖干预组的TGF-β1、Smad2、Smad4的表达水平明显明显高于高糖组、chemerin组。结论 chemerin可以上调TGF-β/smad通路,可能是chemerin促进糖尿病肾病的病理机制。
Objective To investigate the effect of chemerin on the TGF-β / smad pathway and to explore the role of chemerin in the pathogenesis of diabetic nephropathy. METHODS: Rat mesangial cells were cultured in DMEM medium supplemented with DMDM. 30 mmol / L glucose was added into the high glucose group, 0.5 mmol / L chemerin protein was added to the chemerin intervention group, while chemerin + high glucose combined with intervention group 0.5 mmol / L chemerin protein, 30 mmol / L glucose. After 72 hours, the cell pellet was collected and the expression of TGF-β1, Smad2 and Smad4 in each group was detected by realtime-PCR. Results Compared with the control group, the expressions of TGF-β1, Smad2 and Smad4 in high glucose group and chemerin group were significantly higher than those in the control group, while the expressions of TGF-β1, Smad2 and Smad4 in chemerin + high glucose group were significantly higher than those in the control group Sugar group, chemerin group. Conclusion Chemerin can up-regulate the TGF-β / smad pathway and may be the chemerin pathological mechanism of diabetic nephropathy.