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目的研究板栗壳的提取方法以及板栗壳水提物对小鼠耐缺氧作用的影响,为板栗壳的进一步开发和利用提供理论依据。方法用水提法制备板栗壳水提物,各组小鼠给予不同剂量板栗壳水提物连续灌胃7 d,末次给药1 h后进行常压耐缺氧实验。记录存活时间并检测小鼠血清超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量的变化。观察板栗壳水提物对小鼠耐缺氧作用的影响。结果板栗壳水提物可在一定程度上延长小鼠存活时间、实验中用药小鼠血清SOD活力上升、MDA含量下降(均P<0.01);且与用药剂量呈相关性。结论板栗壳水提物能清除自由基,有提高小鼠耐缺氧能力作用。
Objective To study the extraction method of chestnut shell and water chestnut shell extract on hypoxia tolerance in mice and provide a theoretical basis for the further development and utilization of chestnut shell. Methods The aqueous extract of chestnut shell was prepared by water extraction. The mice in each group were given gavage of aqueous extract of chestnut shell at different dosages for 7 days. The rats were subjected to atmospheric hypoxia tolerance test 1 h after the last administration. The survival time was recorded and the activity of serum superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were detected. To observe the effect of chestnut shell aqueous extract on hypoxia tolerance in mice. Results The water chestnut shell extract could prolong the survival time to a certain extent. In the experiment, the serum SOD activity increased and the MDA content decreased (all P <0.01), and was correlated with the dosage. Conclusion chestnut shell water extract can scavenge free radicals, improve the hypoxia tolerance in mice.