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组氨酸是蛋白质组成中的一种碱性氨基酸,测定它对于遗传代谢和营养学等方面的研究有实际的意义。测定组氨酸的方法已有报导。但用库仑滴定法测定组氨酸尚未见报导。我们通过实验,获得了令人满意的结果。 1.仪器与试剂:KD-771B库仑滴定仪(厦门第二分析仪器厂),XYX-2042型自动平衡记录仪(上海自动化仪表二厂),TY型精密pH计(厦门第二分析仪器厂);溴化钾、硝酸钾、氯化钾、无水乙酸钠、乙酸、邻苯二甲酸氢钾、磷酸二氢钾、磷酸氢二钾、浓盐酸和L
Histidine is a basic amino acid in protein composition. It has practical significance for the study of genetic metabolism and nutrition. Methods for the determination of histidine have been reported. However, Coulometric titration of histidine has not been reported. We got satisfactory results through experiments. 1. Instruments and Reagents: KD-771B Coulometric Titrator (Xiamen Second Analytical Instrument Factory), XYX-2042 Automatic Balancing Recorder (Shanghai Automation Instrumentation Plant), TY Precision pH Meter (Xiamen Second Analytical Instrument Factory) ; Potassium bromide, potassium nitrate, potassium chloride, anhydrous sodium acetate, acetic acid, potassium hydrogen phthalate, potassium dihydrogenphosphate, dipotassium hydrogenphosphate, concentrated hydrochloric acid and L