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乳酸脱氢酶是巯基酶。我们以血液乳酸脱氢酶为研究指标,以溴醋酸为巯基酶的抑制剂,对猪蹄爪甲制剂——血液3号(四川内江肉联厂生化制药厂提供)对巯基酶的保护作用进行了研究。采健康成人的末稍循环血稀释50倍供实验用。用比色法测定乳酸脱氢酶活性。 健康人末稍循环血的乳酸脱氢酶活性10247±1852单位％,加入血液3号后测得的酶活性为10110±2027单位％,二者无显著差别(p>0.25),说明血液3号对乳酸脱氢酶活性无直接影响。在溴醋酸的作用下,血液乳酸脱氢酶活性降至522±472单位％,表明溴醋酸对乳酸脱氢酶有强烈的抑制作用(p<0.0005)。血液标本加入溴醋酸后再
Lactate dehydrogenase is a sulfhydryl enzyme. We take the blood lactate dehydrogenase as a research index, bromoacetic acid as a sulfhydryl enzyme inhibitor, the trotter preparation - blood No.3 (Sichuan Neijiang meat factory Biochemical Pharmaceutical Factory) on the sulfhydryl enzyme protection Study. Minimallyssected circulating blood from healthy adults was diluted 50-fold for experimental use. Determination of lactate dehydrogenase activity by colorimetry. Blood lactate dehydrogenase activity in healthy persons was 10247 ± 1852 units%, and the activity of enzyme was 10110 ± 2027% after addition of blood 3, there was no significant difference (p> 0.25), indicating that blood 3 No direct effect on lactate dehydrogenase activity. In the presence of bromoacetic acid, blood lactate dehydrogenase activity decreased to 522 ± 472 units%, indicating that bromoacetic acid strongly inhibited lactate dehydrogenase (p <0.0005). Blood samples after adding bromoacetic acid