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对山东地区海水养殖海域常见抗生素耐药菌及耐药基因分布进行调查,结果表明所调查5个海水养殖区水样中四环素类、磺胺类、β-内酰胺类耐药菌比例显著(p<0.05)高于氯霉素类和喹诺酮类耐药菌;此外用RT-PCR方法对共计15种耐药基因在水样中的丰度测定表明磺胺类(sul、dfra/16S rRNA=10~(-6)~10~(-2))、喹诺酮类(qnr/16S rRNA=10~(-6)~10~(-2))以及四环素类耐药基因(tet/16S rRNA=10~(-7)~10~(-2))在各水样中丰度差异不显著,而氯霉素耐药基因(cata、cmle/16S rRNA=10~(-8)~10~(-2))在不同水样中丰度差异显著,且cata1和cmle1丰度与可培养氯霉素耐药菌比例存在相关线性关系。实验数据说明山东海水养殖区水样中存在一定的抗生素耐药菌和耐药基因污染。
To investigate the distribution of common antibiotic resistant genes and drug resistance genes in mariculture areas in Shandong Province, the results showed that the proportions of tetracyclines, sulfonamides and β-lactams were significant (p < 0.05) were higher than that of chloramphenicol and quinolone resistant strains. In addition, the abundance of 15 drug resistance genes in water samples by RT-PCR method showed that the sulfonamides (sul, dfra / 16S rRNA = 10 ~ ( (Qnr / 16S rRNA = 10 -6 ~ 10 -2) and tet / 16S rRNA = 10 ~ (-2) There was no significant difference in the abundance of water samples between 7 and 10 ~ (-2)), while the chloramphenicol resistance gene (cata, cmle / 16S rRNA = 10 -8 ~ 10 -2) There was a significant difference in abundance in different water samples, and there was a linear relationship between abundance of cata1 and cmle1 and the proportion of resistant chloramphenicol-producing bacteria. Experimental data show that there is a certain degree of antibiotic-resistant bacteria and drug-resistant gene contamination in the water samples of Shandong marine aquaculture area.