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目的:采用D-gal建立衰老小鼠模型,探索合适D-gal浓度,对造模方法进行评价。方法:将60只2月龄雄性Balb/c小鼠随机分为衰老模型组即注射D-gal低剂量150mg/(kg·d)组、注射D-gal高剂量500mg/(kg·d)组)和空白对照组,分别采用D-gal溶液、生理盐水颈背部连续皮下注射6w,进行行为学及体内相关蛋白表达水平检测。结果:与空白对照组比较,衰老模型组小鼠学习记忆能力下降(P<0.05);血清MDA含量增加,SOD、GSH-Px活性降低(P<0.05)。衰老模型组中,注射D-gal低剂量组相关数据更接近自然衰老对照组。结论:通过合适剂量D-gal诱导的方法所建立衰老模型在整体水平上能较好模拟动物自然衰老过程。
OBJECTIVE: To establish an aging mouse model by using D-gal to explore the suitable D-gal concentration and evaluate the method of modeling. Methods: Sixty two-month-old male Balb / c mice were randomly divided into aging model group, D-gal low dose 150mg / (kg · d) group and high dose D-gal 500mg / (kg · d) ) And blank control group, respectively, using D-gal solution, saline subcutaneously on the back of the neck continuous 6w, behavioral and related protein levels in vivo detection. Results: Compared with the blank control group, the learning and memory abilities of aging model group decreased (P <0.05); the content of serum MDA increased and the activities of SOD and GSH-Px decreased (P <0.05). In aging model group, the data of D-gal low-dose injection group was closer to that of natural aging control group. CONCLUSION: The aging model established by the method of D-gal induced by the appropriate dose can better simulate the natural aging process of animals at the overall level.