Isolation of Endophytic Fungi in Daphniphyllum macropodum and Their Antimicrobial Activities

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[Objective] To isolate the endophytic fungi from Daphniphyllum macropodum and to study their antimicrobial activities. [Method] Firstly, endophytic fungi from D. macropodum were isolated by mycelial tip picking method. Then, according to Microbial Taxonomy and morphology method, the endophytic fungi were identified. Lastly, antimicrobial experiments of endophytic fungi were carried out with Oxford cup assay. [Result] 8 different endophytic fungi were obtained from the leaves and the stems of D. macropodum. 1 species of Rhizoctonia, 2 species of Cephalosporium, 1 species of Trichoderma, 2 species of Penicillium, 1 species of Mucor and 1 species of Rhizopus were identified respectively. Among them, metabolites of JR0203 strain had relatively strong bacteriostasis effects. [Conclusion] The endophytic fungi from D. macropodum had certain antimicrobial activities, which needed to be further studied. [Objective] To isolate the endophytic fungi from Daphniphyllum macropodum and to study their antimicrobial activities. [Method] Firstly, endophytic fungi from D. macropodum were isolated by mycelial tip picking method. Then, according to Microbial Taxonomy and morphology method, the endophytic fungi Lastly, antimicrobial experiments of endophytic fungi were carried out with Oxford cup assay. [Result] 8 different endophytic fungi were obtained from the leaves and the stems of D. macropodum. 1 species of Rhizoctonia, 2 species of Cephalosporium, 1 species of Trichoderma, 2 species of Penicillium, 1 species of Mucor and 1 species of Rhizopus were identified respectively. Among them, metabolites of JR0203 strain had relatively strong bacteriostasis effects. [Conclusion] The endophytic fungi from D. macropodum had certain antimicrobial activities, which needed to be further studied.
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