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目的 通过建立A5 4 9人肺腺癌细胞 6 15 SCID小鼠模型 ,评价重度联合免疫缺陷 6 15 SCID小鼠在建立人类肺癌转移模型方面的应用价值。方法 将 1× 10 7A5 4 9细胞接种到 6 15 SCID及SCID小鼠右上肢背部皮下 ,观察成瘤时间、成瘤率、肿瘤生长速度及转移发生。结果 两品系小鼠接种后的成瘤率均为 10 0 % ,6 15 SCID小鼠移植瘤潜伏期较长、生长较缓慢 ,更容易发生转移。结论 6 15 SCID小鼠比SCID小鼠更易于构建人类肺腺癌转移模型 ,对于肺癌转移特性研究具有较大的意义。
OBJECTIVE: To evaluate the value of establishing a model of lung metastasis in human lung cancer by establishing A549 cell line of 6 15 SCID in lung cancer cells. Methods 1 × 10 7A5 4 9 cells were inoculated subcutaneously into the right upper limbs of 6 15 SCID and SCID mice. The tumorigenic time, tumor formation rate, tumor growth rate and metastasis were observed. Results The tumorigenic rates of the two strains of mice after inoculation were both 100%. The transplanted tumors of 6-15 SCID mice had longer incubation period, slower growth and more easily metastasis. CONCLUSIONS: 6 15 SCID mice are easier to construct human lung adenocarcinoma metastasis model than SCID mice, which is of great significance for the study of metastasis characteristics of lung cancer.