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应用培养纤维母细胞方法作细胞水平的老化研究,Haytlick发现培养纤维母细胞三的增殖是有限的,同时又指出,个体最大寿命是与MPD(平均人口倍数)呈较大本关性。近年来,细胞老化的研究己进入分子水平阶段,细胞融合试验的结果阐明了影响老化因子的存在,以及对成长因子受体随年龄增长其反应性发生改变等新见解。这种实验不仅对早衰,而且对其它病,如糖尿病等寿命缩短的阐明也是一个探索的途径。1969年Goldstein等报告培养糖尿病人的皮肤纤维母细胞,其增殖能力降低,但过去对此无统一认识。最近,Archer等对此问题进行了研究,对象限定为糖尿病控制良好的,无明显并发症的IDDM患者,根据病史分<1年、1～5年、6～10年以及>10年四组。从患者
Using the method of culturing fibroblasts for cell-level aging, Haytlick found that the proliferation of cultured fibroblasts is limited and at the same time points out that the maximum lifespan of an individual is of greater relevance to MPD (mean population multiplier). In recent years, cell aging research has entered the molecular stage, the results of the cell fusion test illustrates the existence of aging factors, as well as changes in the responsiveness of growth factor receptors with age, and other new insights. This experiment is not only for premature senility, but also to clarify the shortened life expectancy of other diseases, such as diabetes is also a way to explore. Goldstein et al. Reported in 1969 that cultured human skin fibroblasts from diabetic patients had a decreased ability to proliferate, but there was no uniform understanding in the past. Recently, Archer et al. Studied this issue and defined it as a well-controlled diabetic patient with IDDM who did not have significant morbidity, divided into four groups according to their clinical history of <1 year, 1 to 5 years, 6 to 10 years, and> 10 years. From the patient