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本工作分析了不同形态质粒DNA和未成熟胚高渗透压处理对基因枪小麦转化体系的适用性。高渗处理对瞬间表达和转基因小麦的再生均有明显的促进作用。轰击之前对质粒DNA进行变性处理导致瞬间表达反应大幅度下降,但稳定转化频率(指从100个轰击未成熟胚得到的再生可育转基因植株数)与双链DNA相差不大。使用单链质粒DNA、线性双链质粒DNA和环状双链质粒DNA均可以得到转基因小麦植株。迄今已得到26个不同的转基因冬小麦株系和4个不同的转基因春小麦株系。这些转基因小麦大多数已产生种子,几个春小麦株系已获第二代种子。
This work analyzed the applicability of different forms of plasmid DNA and immature embryos to osmotic transformation of transgenic wheat. Hypertonic treatment promoted the transient expression and the regeneration of transgenic wheat significantly. Denaturation of plasmid DNA prior to bombardment led to a sharp decrease in transient expression responses, but stable transformation frequency (referring to number of regenerated fertile transgenic plants from 100 bombardment of immature embryos) was comparable to that of double-stranded DNA. Transgenic wheat plants can be obtained using single stranded plasmid DNA, linear double stranded plasmid DNA and circular double stranded plasmid DNA. To date, 26 different transgenic winter wheat lines and 4 different transgenic spring wheat lines have been obtained. Most of these GM wheat have produced seeds and several of the spring wheat lines have been given second-generation seeds.