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目的探讨精浆胸苷激酶1(Thymidine kinase 1,TK1)的浓度与精子参数的关系。方法利用增强化学发光法检测无精子症和少弱精子症及已生育健康人精浆TK1的浓度。同时利用计算机辅助精液分析系统分析各实验组精子密度及活力。各实验组精浆酸性磷酸酶、α-葡糖苷酶、精浆果糖和锌的浓度也一并检测。分析精浆TK1浓度变化和精子参数、精浆相关参数的相关性。结果弱精子组(2.41±0.21)及无精子组(2.72±1.16)患者精浆TK1的浓度显著高于正常对照组(1.53±0.22)(P<0.0 1),少精子组(1.60±0.1 3)和少弱精子组(1.68±0.24)患者精浆TK1的浓度与对照组相比差异没有统计学意义(P>0.05)。精浆TK1与精浆生化指标即精浆酸性磷酸酶、α-葡糖苷酶、果糖和锌的浓度都没有显著的相关性(P>0.05)。结论 TK1可能与精子活动质量有一定相关性,高浓度的TK1是引起弱精子症的原因之一,与精子数量的变化关系不密切。TK1在无精子症患者中浓度偏高的原因有待进一步研究。
Objective To investigate the relationship between the concentration of sperm thymidine kinase 1 (TK1) and sperm parameters. Methods Enhanced chemiluminescence was used to detect the concentration of TK1 in azoospermia, oligoasthenospermia and healthy human semen. Simultaneous use of computer-aided semen analysis system analysis of the experimental group sperm density and vitality. The concentration of acid phosphatase, α-glucosidase, fructose and zinc in the experimental group were also detected. Analysis of the changes in the concentration of seminal plasma TK1 and sperm parameters, the relevance of seminal plasma parameters. Results The concentration of TK1 in the seminal plasma of the weak sperm group (2.41 ± 0.21) and the azoospermia group (2.72 ± 1.16) was significantly higher than that of the normal control group (1.53 ± 0.22) (P <0.01) (1.68 ± 0.24), the difference was not statistically significant (P> 0.05) between the concentration of TK1 and the control group. There was no significant correlation between the seminal plasma TK1 and the seminal plasma biochemical indexes, namely, the concentrations of seminal plasma acid phosphatase, alpha-glucosidase, fructose and zinc (P> 0.05). Conclusion TK1 may be related to the quality of sperm motility. High concentration of TK1 is one of the causes of asthenospermia, which is not related to the change of sperm count. The reason for the high concentration of TK1 in patients with azoospermia remains to be further studied.