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应用免疫荧光抗体法或免疫酶组化法(酶一抗酶复合体,简称PAP法)发现某些哺乳类动物(牛、羊、猪和鼠)及人垂体前叶的促肾上腺皮质激素(ACTH)细胞,可分泌ACTH、β-内啡肽、β-MSH(β-促黑色素激素)及β-LPH(β-向脂素等激素);并且证明这些激素定位在该细胞的同一分泌颗粒内(Moon-1973,Pellefier 1977)。在应激状态下,这些激素同时被泌出,并排出量增多(Guillemn 1977)。在暴冷或手术时,血清中的皮质酮、生长激素(GH)及催乳素(PRL)的浓度的增高。现认为这是由于强烈刺激引起机体的急性应激反应,促使垂体同时分泌ACTH、β-内啡肽、β-LPH、β-MSH、GH及PRL等激素
Immunofluorescence antibody or enzyme immunohistochemistry (enzyme-antibody complex, referred to as PAP) found that some mammals (cattle, sheep, pigs and mice) and human anterior pituitary adrenocorticotropic hormone (ACTH ) Cells secreting ACTH, β-endorphin, β-MSH (β-melanin hormone) and β-LPH (β-defensin and other hormones); and proves that these hormones are localized in the same secretory granules of the cell (Moon-1973, Pellefier 1977). Under stress, these hormones are excreted and increased in volume (Guillemn 1977). Serum corticosterone, growth hormone (GH), and prolactin (PRL) concentrations increase during periods of cold or surgery. Now that this is due to a strong stimulus caused by the body’s acute stress response, prompting the pituitary while secreting ACTH, β-endorphin, β-LPH, β-MSH, GH and PRL and other hormones