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目的探讨侵袭性曲霉病与医院环境曲霉污染是否有关。方法对西南医院肝移植病房、脑外科ICU病房和中心ICU病房环境及高危患者鼻腔、咽部和痰液标本进行监测培养.采用随机扩增多态性DNA分析方法对分离自环境和患者体内的黄曲霉进行基因分型研究。结果肝移植病房、脑外科ICU病房、中心ICU病房空气曲霉密度分别12、10.75 cfu/m~3和0。医院环境中5种常见的曲霉依次为黄曲霉、烟曲霉、黑曲霉、杂色曲霉和棒曲霉。RAPD分析显示,脑外科ICU 2例患者体内分离的黄曲霉与病房环境分离的黄曲霉基因型相同;中心ICU病房3例患者体内分离的黄曲霉与病房环境分离的黄曲霉基因型均不相同,但2例患者体内分离的黄曲霉基因型相同。结论肝移植病房、脑外科ICU病房和中心ICU病房存在不同程度曲霉污染,脑外科ICU 2例患者体内分离的黄曲霉和环境中分离的黄曲霉基因型相同。
Objective To investigate whether invasive aspergillosis is related to hospital environmental Aspergillus contamination. Methods The specimens of nasal cavity, pharynx and sputum in the liver transplantation ward, the brain surgery ICU ward and the center ICU ward of Southwest Hospital were monitored and cultivated, and the random amplified polymorphic DNA Aspergillus flavus for genotyping. Results The density of Aspergillus aeruginosa in liver transplantation ward, brain surgery ICU ward and central ICU ward were 12,10.75 cfu / m ~ 3 and 0 respectively. The five common Aspergillus species in hospital environment were Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus versicolor and Aspergillus clavatum. The RAPD analysis showed that the genotypes of Aspergillus flavus isolated from two patients in ICU were the same as those isolated from ward environment. The genotypes of Aspergillus flavus isolated from ward in ICU ward were different from those in ward environment, However, the two isolates had the same genotype of A. flavus in vivo. Conclusion Aspergillus fumigatus was found in the liver transplantation ward, brain surgery ICU ward and central ICU ward. The genotypes of Aspergillus flavus isolated from the body in two patients with ICU in the brain surgery department were the same as those in the environment.