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目的:研究荷叶黄酮对小鼠非酒精性脂肪肝的治疗作用。方法:以复方高脂饮食建立非酒精性脂肪肝小鼠模型,造模第14d,将60只照模鼠随机分为模型组,阳性对照组(易善复150mg/kg),荷叶黄酮低、中、高剂量治疗组(剂量分别是50,100,200mg/kg)。分别观察荷叶黄酮对非酒精性脂肪肝小鼠的肝指数,血清TC、TG、ALT、AST以及肝脏TC、TG含量,观察小鼠肝组织脂质变性程度。结果:荷叶黄酮治疗42d后,非酒精性脂肪肝小鼠肝指数、血清TC、TG、ALT、AST,肝脏TC、TG含量明显降低(P<0.05或P<0.01),并且具有一定的剂量依赖趋势,其中,荷叶黄酮中、高剂量组肝脏TG水平降低显著,与正常组无统计学差异(P>0.05)。光镜观察结果表明,给予荷叶黄酮的小鼠肝组织脂质变性程度改变明显减轻(P<0.01)。结论:荷叶黄酮具有治疗小鼠非酒精性脂肪肝的作用,其作用机制与降低肝脏TG水平有关。
Objective: To study the therapeutic effect of lotus leaf flavonoids on non-alcoholic fatty liver in mice. Methods: A non-alcoholic fatty liver mouse model was established by compound high-fat diet. On the 14th day after modeling, 60 mice were randomly divided into model group, positive control group (Yiwangfu 150mg / kg) , Medium and high dose treatment group (doses were 50,100,200 mg / kg). The liver index, serum TC, TG, ALT, AST, hepatic TC and TG contents in liver of non-alcoholic fatty liver mice were observed respectively to observe the degree of lipid degeneration in mice liver. Results: The liver index, serum TC, TG, ALT, AST, liver TC and TG in non-alcoholic fatty liver mice were significantly decreased (P <0.05 or P <0.01) Dependence trends, among them, lotus leaf flavonoids in high-dose group decreased liver TG levels significantly, with no significant difference between the normal group (P> 0.05). The results of light microscope showed that the degree of lipid degeneration in liver tissue of mice treated with lotus leaf flavonoids was significantly reduced (P <0.01). Conclusion: The lotus leaf flavone has the effect of treating nonalcoholic fatty liver disease in mice, and its mechanism of action is related to the reduction of liver TG level.