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目的:建立内标—标准曲线法定量测定血液中的乙醇含量的气相色谱方法,并与SF/Z JD0107001-2010进行比较。方法:用移液管量取0.20 m L血样及1.00 m L 40.0μg/m L叔丁醇内标工作液加入2.0 m L塑料离心管中,混合均匀,离心后将清液通过0.45μm的无机滤膜过滤后装入2.0 m L进样瓶中密封,应用自动进样器进样,采用ZB-624柱分离样品中的乙醇和叔丁醇,然后采用内标—曲线法定量分析血液中的乙醇含量。结果:内标—标准曲线法定量分析血液中的乙醇含量,乙醇的加标回收率在97.6%~101.0%,相对标准偏差小于2.0%,且重现性好。结论:该法分析血液中的乙醇含量能满足检测工作要求,相较顶空进样—气相色谱法更简单,难度也更小。
Objective: To establish a gas chromatographic method for the quantitative determination of ethanol content in blood by internal standard - standard curve method and compare with SF / Z JD0107001-2010. METHODS: Blood samples of 0.20 m L and 1.00 m L of 40.0 μg / mL tert-butanol internal standard working solution were pipetted into a 2.0 mL plastic centrifuge tube and mixed well. After centrifugation, the supernatant was passed through a 0.45 μm inorganic The membrane was filtered and filled into a 2.0 mL syringe vial and sealed. Samples were injected using an autosampler. The ZB-624 column was used to separate the ethanol and t-butanol from the sample. The internal standard curve was then used to quantify Ethanol content. Results: The content of ethanol in the blood was quantitatively determined by internal standard - standard curve method. The recoveries of ethanol were 97.6% -101.0% with relative standard deviations less than 2.0% and reproducibility was good. Conclusion: The method of analysis of ethanol content in the blood can meet the testing requirements, compared with the headspace injection - gas chromatography is more simple and less difficult.