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目的 探讨动脉血管平滑肌细胞凋亡在动脉粥样硬化发生、发展中的作用及细胞凋亡与 p5 3、bcl- 2、c- m yc表达的内在联系 ,为动脉粥样硬化的防治提供新的思路。 方法 (1)选择因动脉粥样硬化住院手术患者 2 0例为观察组 ,因意外伤害手术患者的正常动脉 10例为对照组。两组标本均经病理切片证实。 (2 )采用末端脱氧核苷酸转移酶介导的d UTP缺口末端标记 (TU NEL)技术比较两组动脉平滑肌细胞凋亡的差异。 (3)应用免疫组织化学方法检测动脉平滑肌细胞 c- myc、bcl- 2和 p5 3等凋亡相关基因表达的差异。 (4 )应用透射电镜观察动脉粥样硬化及对照组动脉平滑肌细胞形态学差异。 结果 动脉粥样硬化组动脉平滑肌细胞凋亡率明显增加 ,C- m yc蛋白、P5 3蛋白表达明显升高 ,Bcl- 2蛋白表达明显降低 (P<0 .0 0 1)。 结论 动脉平滑肌细胞凋亡与增生共同参与动脉粥样硬化的发生、发展。c- myc基因和 p5 3基因促进细胞凋亡 ,而 bcl- 2基因抑制细胞凋亡
Objective To investigate the role of apoptosis of arterial smooth muscle cells in the development and progression of atherosclerosis and the relationship between apoptosis and the expression of p5 3, bcl-2 and c-m yc and to provide a new method for the prevention and treatment of atherosclerosis Ideas. Methods (1) Twenty patients undergoing atherosclerosis hospitalization were selected as the observation group and 10 normal arteries of patients undergoing accidental injury as the control group. Two groups of specimens were confirmed by biopsy. (2) The difference of apoptosis of arterial smooth muscle cells between two groups was compared by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TU NEL). (3) The expression of apoptosis-related genes such as c-myc, bcl-2 and p5 3 in arterial smooth muscle cells were detected by immunohistochemistry. (4) The morphology of atherosclerosis and control group arterial smooth muscle cells were observed by transmission electron microscope. Results The apoptotic rate of atherosclerosis group was significantly increased, the expression of C-yc protein and P53 protein was significantly increased, and the expression of Bcl-2 protein was significantly decreased (P <0.01). Conclusion Apoptosis and proliferation of arterial smooth muscle cells participate in the development of atherosclerosis. c-myc gene and p5 3 gene promote apoptosis, while bcl-2 gene inhibits apoptosis