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蜡蚧轮枝菌用湿地松粉蚧连续复壮２～３次后，用其防治湿地松粉蚧，杀虫效果可提高１２．３％以上，筛选出轮枝菌复合剂的最优配方为轮枝菌０．３亿／ｍｌ加机油乳剂１：２８．６（机油乳剂：水）及适量增效剂、悬浮剂及轮枝菌０．２５亿／ｍｌ加机油乳剂１：８０及增效剂。悬浮剂，它们室内杀虫效果分别为９２．８％和８３．５％。林间采用轮枝菌１５１亿／ｈｍ２加机油乳剂０．７５ｋｇ／ｈｍ２和轮枝菌１５万亿／ｈｍ２加机油乳剂３．７５ｋｇ／ｈｍ２，喷洒量为１０ｋｇ／ｈｍ２，越冬代粉蚧（成虫）的死亡率分别为４８．５％和７１．２％，第一代粉蚧（若虫、成虫）的死亡率分别为９９．３％和９２．７％。越冬后期喷菌，第一代的虫口密度比未喷菌区下降５２．７％；采用液、固双相发酵工艺生产的轮枝菌含孢量达５５亿／ｇ，比前人用高梁、小米作培养基密封培养的产孢量（４．１亿／ｇ）提高了１２．４倍，成本为３．３８元／ｋｇ，此工艺流程可以推广应用。
After the Verticillium lecanii was rejuvenated continuously for 2 ~ 3 times using the slash pine slash, the insecticidal effect could be increased by 12.3% Mycobacterium 0.3 billion / ml plus oil emulsion 1: 28.6 (oil emulsion: water) and the amount of synergist, suspending agent and rotifers 250000000 / ml plus oil emulsion 1:80 and synergist . Suspension, indoor insecticidal effect of them were 92.8% and 83.5%. In the forest, 15.1 billion / hm2 of rotavirus plus 0.75 kg / hm2 of oil emulsion and 15 trillion / hm2 of rotavirus plus 3.75 kg / hm2 of oil emulsion were sprayed at a rate of 10 kg / hm2, ) Mortality rates were 48.5% and 71.2% respectively. The mortality rates of the first generation mealybugs (nymphs and adults) were 99.3% and 92.7%, respectively. After the late winter spraying, the population density of the first generation was 52.7% lower than that of the non-sprayed area. The spore yield of Verticillium sp. Produced by liquid-solid two-phase fermentation was 5.5 billion / g, Millet as a medium sealed culture sporulation (410 million / g) increased by 12.4 times, the cost of 3.38 yuan / kg, this process can be promoted.