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目的研究喉癌癌变过程中ＣｙｃｌｉｎＥ和ｐ５３表达的临床病理学意义。方法用免疫组化检测２０例喉正常粘膜、４０例不典型增生病变和６０例喉癌组织中ＣｙｃｌｉｎＥ和ｐ５３的表达。结果ＣｙｃｌｉｎＥ和ｐ５３阳性表达均定位于细胞核。在喉癌癌变过程中，喉正常粘膜、不典型增生病变和喉癌中ＣｙｃｌｉｎＥ阳性表达率分别为５．０％（１／２０）、２０．０％（８／４０）和４５．０％（２７／６０）（Ｐ＜０．００１）。ｐ５３阳性表达率分别为０、３０．０％（１２／４０）和６１．７％（３７／６０）（Ｐ＜０．００１）。淋巴结转移组ＣｙｃｌｉｎＥ阳性表达率明显高于非淋巴结转移组；ＣｙｃｌｉｎＥ和ｐ５３阳性表达显著性相关（Ｐ＜０．０５）。结论ＣｙｃｌｉｎＥ和ｐ５３异常表达可能是喉癌发生中早期分子事件，对喉癌发展起重要作用。
Objective To investigate the clinicopathological significance of CyclinE and p53 expression in carcinogenesis of laryngeal carcinoma. Methods The expressions of CyclinE and p53 in 20 normal laryngeal mucosa, 40 atypical hyperplasia lesions and 60 laryngeal carcinoma tissues were detected by immunohistochemistry. Results The positive expression of CyclinE and p53 localized in the nucleus. The positive expression rate of CyclinE in laryngeal normal mucosa, atypical hyperplasia lesions and laryngeal carcinoma was 5.0% (1/20), 20.0% (8/40) and 45.0% 27/60) (P <0.001). The positive rates of p53 were 0, 30.0% (12/40) and 61.7% (37/60), respectively (P <0.001). The positive expression rate of CyclinE in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group (P <0.05). Conclusion The abnormal expression of CyclinE and p53 may be the early molecular event in the development of laryngeal carcinoma and plays an important role in the development of laryngeal carcinoma.