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目的：对国人神经生长因子β亚基（βＮＧＦ）ｃＤＮＡ 进行克隆及序列分析，为研究βＮＧＦ 的生理功能及其在临床应用提供基础与手段。方法：从人海马组织分离，纯化总ＲＮＡ，直接进行逆转录，用ＰＣＲ 扩增βＮＧＦ 的ｃＤＮＡ 得到６５０ｂｐ 的ＤＮＡ 片段，利用Ｔ－ Ａ 克隆载体法，制备重组质粒，克隆βＮＧＦｃＤＮＡ。结果：βＮＧＦ 的ｃＤＮＡ 全序列１０７４ ｂｐ ，其蛋白编码为６３０ ｂｐ ，由２１２ 个氨基酸组成，Ｎ 端为信号肽，中间区为前肽，Ｃ 端为成熟肽。结论：国人βＮＧＦ 序列分析结果与ｇｅｎｅ ｂａｎｋ 中的βＮＧＦ 序列完全一致，与外国人没有区别
Objective: To clone and sequence the cDNA of human nerve growth factor β subunit (βNGF) in order to study the physiological function of βNGF and provide the basis and means for its clinical application. METHODS: Total RNA was isolated and purified from human hippocampus. Reverse transcription was performed directly. CDNA of βNGF was amplified by PCR to obtain a 650 bp DNA fragment. The recombinant plasmid was cloned by T-A cloning vector and cloned βNGF cDNA. RESULTS: The cDNA of βNGF was 1074 bp in length and its protein was 630 bp in length. It consisted of 212 amino acids with N-terminal as signal peptide, intermediate region as pro-peptide and C-terminal as mature peptide. Conclusion: The results of Chinese βNGF sequence analysis are in good agreement with the βNGF sequence of gene bank, which is indistinguishable from foreigners