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目的：阐述ＩＬ－６与胰岛功能或胰岛病变的可能关系。方法：采用常规消化方法分离人胚胎胰岛并进行原代培养，培养上清进行ＩＬ－６、胰岛素、胰高血糖素活性测定和ＩＬ－６ＭｃＡｂ中和实验，同时对胚胎胰岛进行体外葡萄糖刺激实现。结果：原代培养的胚胎胰岛在４０ｈ内ＩＬ－６分泌呈上升趋势（３７～１５３ｍＵ／胰岛），每２４ｈ重新换取新培养液作为一个分泌周期，ＩＬ－６分泌以第１周期最高（１０６ｍＵ／胰岛），体外连续培养ＩＬ－６的分泌以１ｄ最高（１４５ｍＵ／胰岛），用ＩＬ－６ＭｃＡｂ可以阻断胰岛培养上清中ＩＬ－６的活性；胰岛素、胰高血糖素的分泌周期与ＩＬ－６不同；葡萄糖刺激明显促进ＩＬ－６的分泌，其分泌趋势与胰岛素分泌一致。结论：原代培养的人胚胎胰岛在无任何刺激情况下可自发分泌ＩＬ－６，而且ＩＬ－６的分泌有自己的分泌环路。
Objective: To elucidate the possible relationship between IL-6 and pancreatic islet function or islet lesion. Methods: The islets of human embryos were isolated by conventional digestion and cultured in primary culture. The supernatant was cultured for determination of IL-6, insulin, glucagon activity and IL-6McAb neutralization, and in vitro glucose stimulation of embryonic islets. RESULTS: The primary culture of embryonic islets showed an upward trend of IL-6 secretion (37 ~ 153mU / islet) within 40 hours. Fresh medium was replaced every 24 hours as a secretory cycle. IL-6 secretion was the highest in the first cycle (106mU / The secretion of IL-6 in continuous culture in vitro was the highest at 1d (145mU / islet). The IL-6 activity in islet culture supernatant was blocked by IL-6McAb. The secretion of insulin and glucagon was correlated with IL- 6 different; glucose stimulation significantly promote the secretion of IL-6, the secretion of insulin secretion and the same trend. CONCLUSION: Primary cultured human embryonic islets spontaneously secrete IL-6 without any stimulation and IL-6 secretion has its own secretory loop.