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目的探讨凋亡相关基因Livin mRNA在骨肉瘤的表达及其与骨肉瘤发生发展的关系。方法应用逆转录-聚合酶链反应(RT-PCR)检测47例骨肉瘤组织中Livin mRNA的表达情况,结合临床资料进行分析。结果 Livin mRNA在经病理证实的20例肿瘤外5cm的正常骨组织中均未检出,47例骨肉瘤组织中29例表达阳性,Livin mRNA的表达与Enneking分期有关(P<0.05),而与肿瘤部位和Dahlin病理分型无关(P>0.05)。结论 Livin mRNA在骨肉瘤组织中的特异性表达可能在骨肉瘤的发生发展中起重要作用。阻断Livin mRNA的表达可为骨肉瘤的治疗提供新的途径。
Objective To investigate the expression of apoptosis-related gene Livin mRNA in osteosarcoma and its relationship with the development of osteosarcoma. Methods The expression of Livin mRNA in 47 cases of osteosarcoma was detected by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed with clinical data. Results Livin mRNA was not detected in 5 cm of normal bone tissue of 20 cases confirmed by pathology. The positive expression of Livin mRNA in 47 cases of osteosarcoma was correlated with Enneking staging (P <0.05) Tumor site and Dahlin pathological type has nothing to do (P> 0.05). Conclusion The specific expression of Livin mRNA in osteosarcoma tissue may play an important role in the development of osteosarcoma. Blocking the expression of Livin mRNA may provide a new approach for the treatment of osteosarcoma.