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目的:建立同时测定传统中药山银花中绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙含量的HPLC分析方法。方法:采用Phenomenex Luna C18(250 mm×4.6 mm,5μm)色谱柱,流动相0.4%醋酸(A)-乙腈(B),梯度洗脱(0~12 min,80%A;12~13min,80%A→67%A;13~30 min,67%A→65%A;30~35 min,65%A→80%A),流速0.5 mL.min-1;UV检测:检测绿原酸,λ=326nm;ELSD检测:检测灰毡毛忍冬皂苷乙和川续断皂苷乙,雾化室温度88℃,漂移管温度108℃,N2气流量1.0 L.min-1。结果:线性范围内3个化合物呈良好的线性关系(R2>0.9959),加样回收率在99%~101%之间(n=6),该方法精密度、稳定性和重复性良好,RSD<2%。结论:该方法能够方便、准确地测定山银花中绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。
OBJECTIVE: To establish an HPLC method for the simultaneous determination of chlorogenic acid, pterostilbene saponins B and nigrostrobin B in traditional Chinese medicinal herbs. METHODS: The mobile phase consisted of 0.4% acetic acid (A) -acetonitrile (B) and gradient elution (0-12 min, 80% A; 12-13 min, 80) using a Phenomenex Luna C18 (250 mm × 4.6 mm, % A → 67% A; 13-30 min 67% A → 65% A; 30-35 min, 65% A → 80% A) at a flow rate of 0.5 mL.min-1 UV detection: λ = 326nm; ELSD detection: Detection of pterostilbene saponins B and endochitosan B, atomization room temperature 88 ℃, drift tube temperature 108 ℃, N2 gas flow 1.0 L.min-1. Results: The linear range of the three compounds showed a good linear relationship (R2> 0.9959) with the recoveries of 99% ~ 101% (n = 6). The method showed good precision, stability and repeatability. The RSD <2%. Conclusion: This method can easily and accurately determine the content of chlorogenic acid, pterostilbene saponins B and Duanchuan Saponin B in.