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树突状细胞(dendritic cells,DC)作为抗原提呈细胞,具有独特的活化初始型T细胞的能力.白细胞介素-12(IL-12)可促进NK,T细胞的增殖及杀伤活性,以及初始型T细胞向CD4 Thl细胞的分化.本研究采用GM-CSF+TNF-α诱导小鼠骨髓细胞产生的DC,观察了在DC成熟过程中IL-12的产生,以及在DC成熟过程中应用IL-12对DC的表型和功能的影响.采用FLISA法检测了GM-CSF+TNF-α诱生的DC产生IL-12的水平,结果显示,第5天时,10~6DC培养上清中IL-12 P40的水平为120pg/ml,IL-12P70为20pg/ml;至第8天时,lL-12P40和IL-12 P70水平均升高,分别为150pg/ml和24pg/ml.在DC培养的第3～7天,加入IL-12(25ng/ml),可观察到DC在混合白细胞反应过程中,刺激T细胞增殖的能力显著增强;检测DC的表型,可观察到应用IL-12后,CD80的表达水平显著提高,而CD40,CD54,CD80,MHC-Ⅱ等表面分子则无明显变化.上述
As an antigen presenting cell, dendritic cells (DCs) have a unique ability to activate naive T cells, and interleukin-12 (IL-12) can promote the proliferation and cytotoxicity of NK and T cells and Differentiation of naive T cells into CD4 Th1 cells.In this study, we used DCs induced by GM-CSF and TNF-α to produce mouse bone marrow cells and observed the production of IL-12 during DC maturation and its application in DC maturation The effect of IL-12 on the phenotype and function of DC DC-induced IL-12 production by DC-GM-CSF + TNF-α was detected by FLISA method. The results showed that on the 5th day, Levels of IL-12 P40 were 120 pg / ml and IL-12 P70 was 20 pg / ml; both IL-12 P40 and IL-12 P70 levels were increased by 150 pg / ml and 24 pg / ml, respectively, On day 3 to 7, IL-12 (25ng / ml) was observed in DC mixed leukocyte response to stimulate T cell proliferation was significantly enhanced; detection of DC phenotype, the observed IL-12 , The level of CD80 expression increased significantly, while the surface molecules such as CD40, CD54, CD80, MHC-Ⅱ showed no significant changes.