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为了明确中药复方对隐性乳房炎患病奶牛血清中相关酶活性变化的影响,试验选用穿山甲、芙蓉花、金银花、连翘、蒲公英、通草、丝瓜络、紫花地丁及王不留行等中药按照一定比例组方,采用单纯的水煎浓缩法提取有效成分,对患乳房炎泌乳奶牛进行治疗,以健康牛为对照,对健康牛和用药前后乳房炎奶牛血清中乳酸脱氢酶(LDH)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)相关指标进行了测定与分析。结果表明:隐性乳房炎组奶牛在用药第21天时分别使血清中的LDH和ALT活性下降到正常水平,与对照组差异不显著(P>0.05);隐性乳房炎组奶牛血清AST活性在用药第15,21天时降到正常水平,与对照组差异不显著(P>0.05)。说明中药复方对奶牛乳房炎有较好的防治效果,能够修复奶牛受损的乳腺上皮细胞,降低受损细胞产生的酶含量,具有一定的提高患病奶牛机体免疫机能的作用。
In order to clarify the effect of traditional Chinese medicine compound on the changes of related enzyme activities in the serum of recessive mastitis sick cow, we chose Pangolin, Hibiscus, Honeysuckle, Forsythia, Dandelion, Tongcao, Loofah, Viola yedogensis, According to a certain proportion of prescriptions, pure decoction concentration method was used to extract the active ingredients, to treat mastitis lactating dairy cows, healthy cattle as a control, lactating dehydrogenase (LDH) , Alanine aminotransferase (ALT), aspartate aminotransferase (AST) related indicators were measured and analyzed. The results showed that the serum levels of LDH and ALT decreased to normal level in the recessive mastitis group at 21 d after administration, respectively, with no significant difference from the control group (P> 0.05) On the 15th and 21st day of treatment, the levels dropped to normal levels, with no significant difference (P> 0.05). It shows that Chinese herbal compound has better control effect on cow mastitis, can repair damaged mammary epithelial cells and reduce the enzyme content in damaged cells, and has the effect of improving immune function of sick cow.