本文报道了分离纯化猪血浆纤维粘连蛋白(fibronectin,FN)的方法及其对培养细胞的作用。根据 FN 与明胶和肝素有亲和性的特点,用偶联有明胶和肝素的 Sepharose 4B 亲和层析柱,我们首次从猪血浆中纯化得到大量 FN。将此 FN 涂于瓶底(2μg/cm~2)或加入到培养基中(4μg/ml),观察了 This article reports the method of isolation and purification of porcine plasma fibronectin (FN) and its effect on cultured cells. According to the affinity of FN with gelatin and heparin, we first purified a large amount of FN from porcine plasma with Sepharose 4B affinity chromatography coupled with gelatin and heparin. This FN was applied to the bottom of the bottle (2 μg / cm 2) or added to the medium (4 μg / ml)