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SARS冠状病毒是人的严重急性呼吸综合征的病原体。根据对其他种类冠状病毒的研究结果 ,膜蛋白 (M蛋白 )是病毒主要的结构蛋白 ,重组M蛋白可被用来作为抗原检测对应冠状病毒的感染和制备疫苗。SARS病毒M蛋白基因克隆到原核表达载体pMAL cRI中 ,利用N端和C端分别融合麦芽糖结合蛋白 (maltosebindingprotein和MxeGyrAinteinCBD的策略 ,在大肠杆菌中初步表达了重组M蛋白 ,并通过Western印迹和质谱对蛋白质进行了鉴定。重组蛋白质经亲和层析得到了部分纯化 ,纯化后的蛋白质将用于功能研究与诊断试剂盒的研制。
SARS coronavirus is the causative agent of severe acute respiratory syndrome in humans. Membrane proteins (M proteins) are the major structural proteins of viruses and recombinant M proteins can be used as antigens to detect coronavirus infections and to prepare vaccines, based on the findings of other types of coronaviruses. The SARS virus M protein gene was cloned into the prokaryotic expression vector pMAL cRI. Recombinant M protein was initially expressed in E. coli by the fusion of maltose binding protein (MxeGyrAinteinCBD) on the N terminus and C terminus, respectively. Western blot and mass spectrometry The protein was identified.The recombinant protein was partially purified by affinity chromatography, and the purified protein will be used in the development of functional assay and diagnostic kit.