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目的 探讨细胞增殖和凋亡状态与皮肤血管瘤发生和发展的机制。方法 采用免疫组织化学方法和末端脱氧核苷酸转移酶介导的末端标记技术 (TUNEL) ,检测 40例不同时期婴幼儿皮肤血管瘤及 2 0例周围正常皮肤组织内皮细胞增殖和凋亡状态。结果 2 0例增生期皮肤血管瘤、2 0例退化期皮肤血管瘤及 2 0例正常皮肤组织平均增殖指数分别为 60 .5 8、1.47及 1.49,经配对t检验差异具有高度显著性 (P <0 .0 0 1)。 2 0例增生期皮肤血管瘤、2 0例退化期皮肤血管瘤及2 0例正常皮肤组织平均平均凋亡指数分别为 0 .0 97、1.0 2 1及 1.0 46,经配对t检验差别具有高度显著性 (P <0 .0 0 1)。结论 皮肤血管瘤的血管内皮细胞异常增生和凋亡在血管瘤的发生、发展和退化过程中起着重要作用 ,为临床治疗血管瘤提供新的思路。
Objective To investigate the mechanism of cell proliferation and apoptosis and the occurrence and development of cutaneous hemangiomas. Methods Immunohistochemistry and terminal deoxynucleotidyl transferase mediated terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL) were used to detect the proliferation and apoptosis of endothelial cells in 40 cases of infantile cutaneous hemangiomas and 20 cases of normal peripheral dermal tissues. Results The average proliferative index of 20 cases of proliferative cutaneous hemangiomas, 20 degenerative cutaneous hemangiomas and 20 normal skin tissues were 60.58, 1.47 and 1.49, respectively, which were highly significant by paired t test (P <0 0 0 1). 20 cases of hyperplastic cutaneous hemangiomas, 20 cases of degenerative cutaneous hemangiomas and 20 normal skin tissue average average apoptotic index were 0. 97,1.0 2 1 and 1.0 46, respectively, by the paired t test difference with a high degree Significance (P <0. 001). Conclusion The abnormal proliferation and apoptosis of vascular endothelial cells in cutaneous hemangiomas play an important role in the development, progression and degeneration of hemangiomas, and provide new ideas for clinical treatment of hemangiomas.