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目的:探讨β七叶皂苷钠对烫伤后休克期大鼠血管通透性和组织水肿情况的影响,并观察了β七叶皂苷钠对创周组织微血管结构的影响。方法:将SD大鼠分为正常对照(NC)组,烫伤对照(SC)组和β七叶皂苷钠(AC)组。用电烫仪对SC组和AC组大鼠制作深Ⅱ°烫伤模型(20%TBSA)。烫伤前30min,采用尾静脉注射的方法对AC组大鼠给予β七叶皂苷钠(1.8mg·kg-1),NC组和AC组大鼠分别注射等量0.9%氯化钠注射液。应用改良伊文思蓝渗出法测定了烫伤后早期72h内创周和空肠血管通透性,并测定了组织含水量变化,采用硝酸镧示踪电镜技术观察了创周组织微血管结构的变化。结果:与SC组相比,β七叶皂苷钠可以明显抑制烫伤后早期血管通透性增高,减轻组织水肿发生,并能减轻创周组织微血管结构的的病理性改变。结论:烫伤后早期应用β七叶皂苷钠可以明显减轻烫伤后休克期组织内外渗加剧,因此对烧伤后早期休克及并发症的预防和治疗有重要意义。
OBJECTIVE: To investigate the effects of sodium aescinate on vascular permeability and tissue edema in shocked rats after scald injury, and to observe the effect of sodium aescinate on the microvessel structure during the wounded period. Methods: SD rats were divided into normal control (NC) group, scald control (SC) group and β-apeptin sodium (AC) group. A deep II° scald model (20% TBSA) was made on SC and AC rats using an electric iron. Thirty minutes before burn, rats in AC group were given sodium aescinate (1.8 mg·kg-1) by tail vein injection. Rats in NC group and AC group were injected with equal volume of 0.9% sodium chloride injection. The Evans blue exudation method was used to measure the permeability of perivascular jejunum and jejunum within 72 hours after scald, and the change of tissue water content was determined. The change of microvascular structure in the periwound tissue was observed by lanthanum nitrate tracer electron microscope. RESULTS: Compared with SC group, β-aescinate significantly inhibited the increase of vascular permeability at early stage of burns, reduced the occurrence of tissue edema, and reduced the pathological changes of microvascular structure in perinuclear tissue. Conclusion: The early application of β-aescinate after scald can significantly reduce the exacerbation of tissue and external hemorrhage during the shock period after scalding. Therefore, it is of great significance for the prevention and treatment of early shock and complications after burn.