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The difference of sensitivity to indole-3-acetic acid (IAA) combined with horseradish peroxidase (HRP) in K562 and BXPC-3 cells was investigated.The cell proliferation was determined by MTT assay.The cell cycle and apoptosis of K562 and BXPC-3 cells were examined by a fluorescence flow cytometer (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) respectively. The experimental results show that IAA and HRP could inhibit BXPC-3 cell proliferation greatly compared with K562 cell during the first 48 h. The cell cycle was arrested predominantly at G2/M phase in K562 and BXPC-3 cells. The cell apoptosis of K562 and BXPC-3 was induced by IAA/HRP. There was a significant difference between the two cell lines since BXPC-3 cells were more sensitive than K562 cells by treatments with combination of IAA and HRP.
The difference of sensitivity to indole-3-acetic acid (IAA) combined with horseradish peroxidase (HRP) in K562 and BXPC-3 cells was investigated. The cell proliferation was determined by MTT assay. The cell cycle and apoptosis of K562 and BXPC- 3 cells were examined by a fluorescence flow cytometer (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) respectively. The experimental results show that IAA and HRP could inhibit BXPC-3 cell proliferation greatly compared with K562 cell during the first 48 h. The cell cycle was arrested predominantly at G2 / M phase in K562 and BXPC-3 cells. The cell apoptosis of K562 and BXPC-3 was induced by IAA / HRP. There was a significant difference between the two cell lines since BXPC-3 cells were more sensitive than K562 cells by treatments with combination of IAA and HRP.