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目的:建立人尿中碘含量的酶标仪自动比色测定方法。方法:采用酶标仪自动比色测定法测定人尿中碘含量，对该方法的线性范围、检测限、精密度进行验证；与国家卫生行业标准砷铈催化分光光度法的尿碘测定结果进行比较分析。结果:酶标仪自动比色测定法碘标准曲线的线性范围为0 ~ 300 μg/L，线性相关系数（n r）范围为- 0.999 5 ~ - 0.999 2，检出限为6.5 μg/L；低、中、高3种碘浓度尿样的相对标准偏差（n RSD）均 0.05）；酶标仪自动比色测定法的检测速率是砷铈催化分光光度法的7.5倍。n 结论:酶标仪自动比色测定法应用于尿碘含量测定具有合理的检测线性范围，精密度好，准确度高；且自动化快速比色提高了分析速度，适用于大批量样本检测。“,”Objective:To establish an enzyme-labeled instrument automatic colorimetric method for determination of iodine content in human urine.Methods:The enzyme-labeled instrument automatic colorimetric method was used to determine the iodine content in human urine. The linear range, detection limit and precision of the method were verified. And the urine iodine test results were compared with the results tested by national health industry standard as arsenic-cerium catalytic spectrophotometry.Results:The linear range of iodine standard curve of the enzyme-labeled instrument automatic colorimetric method was 0 - 300 μg/L, the linear correlation coefficient ( n r) was - 0.999 5 to - 0.999 2, and the detection limit was 6.5 μg/L. The relative standard deviation ( n RSD) of urine samples with low, medium and high iodine concentration were all 0.05); and the detection speed of the enzyme-labeled instrument automatic colorimetric method was 7.5 times of the arsenic-cerium catalytic spectrophotometry.n Conclusion:The enzyme-labeled instrument automatic colorimetric method has a reasonable linear range, good precision and high accuracy in determination of urinary iodine content, and the enzyme-labeled instrument automatied fast colorimetry has improved the analysis speed, it is suitable for detection of large quantities of samples.