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目的探讨5-aza-CdR对食管鳞癌甲基化基因的影响。方法应用人类全基因组寡核苷酸微阵列芯片,荧光双交换法检测5-aza-CdR干预的食管鳞癌细胞Eca-109与正常培养的Eca-109细胞中的差异表达基因,并进行生物信息学分析。结果经统计学分析,共筛选获得384个差异表达基因,其中303个基因表达上调,81个基因表达下调;差异基因的功能涉及信号转导、物质合成代谢、细胞周期、细胞增殖、细胞凋亡、DNA转录、DNA复制、DNA修复、氧化还原、物质运输、免疫反应等;上调表达基因中有138个基因分别含有1～5个CpG岛序列,占总上调基因的45.54%。结论5-aza-CdR可以影响食管鳞癌细胞中基因异常的甲基化修饰,调控肿瘤细胞的异常凋亡和分化,为进一步研究食管鳞癌致病分子机制,提供表观遗传学的依据。
Objective To investigate the effect of 5-aza-CdR on methylation status of esophageal squamous cell carcinoma. Methods Human genome-wide oligonucleotide microarrays were used to detect the differentially expressed genes in esophageal squamous carcinoma cell line Eca-109 and normal cell line Eca-109 by fluorescence double exchange method. Bioinformatics Analysis. Results A total of 384 differentially expressed genes were screened, of which 303 genes were up-regulated and 81 genes were down-regulated. The functions of these genes involved in signal transduction, substance synthesis and metabolism, cell cycle, cell proliferation, apoptosis , DNA transcription, DNA replication, DNA repair, redox, substance transport, immune response and so on. Up-regulated genes contained 138 CpG island sequences, accounting for 45.54% of all up-regulated genes. Conclusions 5-aza-CdR can affect the abnormal methylation of esophageal squamous cell carcinoma cells and regulate the abnormal apoptosis and differentiation of esophageal squamous cell carcinoma cells. It provides epigenetic basis for further study on the pathogenesis of esophageal squamous cell carcinoma.