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NDR1(nonrace-specific disease resistance)基因在植物系统获得性抗性的信号传导途径中起着重要的作用,过量表达该基因的拟南芥对不同病原菌的抗性都有提高。利用PCR技术首次克隆了拟南芥Wassilewskija生态型的NDR1基因,与Columbia生态型相比,该基因共有7处碱基不同,引起编码氨基酸变化4处。构建了植物高效表达载体,利用农杆菌介导法转化烟草,经PCR和Southern鉴定,外源基因已整合到植物基因组中。随机挑选10个转基因株系进行抗病性分析,其中3个株系对赤星病和晚疫病的抗性明显增强。
The NDR1 (nonrace-specific disease resistance) gene plays an important role in the signal transduction pathway of acquired resistance in plant system. The resistance of Arabidopsis thaliana overexpressing this gene to different pathogenic bacteria is increased. The NDR1 gene of Arabidopsis Wassilewskija ecotype was cloned by PCR for the first time. Compared with Columbia ecotype, the NDR1 gene of Arabidopsis thaliana shared a total of 7 different bases and caused 4 amino acid changes. The plant expression vector was constructed and Agrobacterium-mediated transformation of tobacco was carried out. The foreign genes were integrated into plant genome by PCR and Southern analysis. Ten transgenic lines were randomly selected for disease resistance analysis, of which three lines were significantly resistant to brown spot and late blight.