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目的:观察固本止咳颗粒对PM2.5致肺损伤模型小鼠肺功能、肺组织形态学的影响,探讨中医“正气卫外”“治未病”理论对PM2.5导致肺损伤的干预和治疗作用。方法:采用鼻腔滴注PM2.5悬液的方法建立PM2.5致小鼠肺损伤模型,实验分为正常对照组、模型对照组、固本止咳中药治疗组;运用动物肺功能仪及HE染色法观察固本止咳颗粒干预PM2.5致肺损伤模型小鼠前后肺功能和肺组织形态学的变化。结果:鼻腔滴注PM2.5悬液成功建立肺损伤模型,模型对照组肺功能明显下降,固本止咳中药治疗组肺功能较模型对照组明显改善(P<0.05),模型对照组肺组织HE染色结果主要为炎性改变,固本止咳中药治疗组病理改变较模型对照组明显改善。结论:固本止咳颗粒可以降低肺损伤小鼠吸气及呼气阻力,提高肺顺应性,并减轻肺组织及气管周围炎性细胞浸润,改善肺功能状态,与中医“治未病”思想相符。
Objective: To observe the effects of Guben Zhike Granule on lung function and lung histomorphology in lungs of PM2.5 -induced model mice and to explore the effect of Gengben Zhike Granules Injury interventions and therapeutic effects. Methods: PM2.5-induced lung injury model was established by intranasal instillation of PM2.5 suspension in mice. The experiment was divided into normal control group, model control group and Guben Cough-Relieving Chinese herb treatment group. Animal lung function test and HE staining To observe the changes of lung function and lung histomorphology of Guben Cough Relief Granule before and after the intervention of PM2.5 induced lung injury model mice. Results: Lung injury model was successfully established by intranasal instillation of PM2.5 suspension. The pulmonary function of the model control group was significantly decreased. The lung function of the Guben Cough-relieving traditional Chinese medicine treatment group was significantly improved compared with the model control group (P <0.05) Staining results mainly for inflammatory changes, Guben Cough Chinese medicine treatment group pathological changes than the model control group was significantly improved. Conclusion: Guben Zhike Granule can reduce inspiratory and expiratory resistance in mice with lung injury, improve lung compliance, and reduce infiltration of inflammatory cells in the lung tissue and trachea to improve lung function status, and Chinese medicine “cure disease” The same idea.