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采用高效液相色谱-飞行时间质谱联用(HPLC-Q-TOF/MS)的代谢组学方法研究了藏药成分。以牦牛作为模式生物,血液样品和藏药去包灵1号经处理后进样分析,利用M arkerview对采集的谱图进行提取、过滤,采用主成分分析(PCA)进行数据分析。结果表明,去包灵1号与不同剂量组牦牛血液中存在共存成分,高剂量组、低剂量组、阳性药组和对照组的代谢轮廓有显著差别,对各组分离贡献大的化合物进行质谱信息匹配,得到80个特征离子,经Metlin和Massbank数据库检索,在复方藏药中,初步鉴定得到8个物质(3-乙基-1,2-环戊二酮、十二烯酸、鹿尾草碱、甲基癸酸、十一烯酸、亚甲二氧基黄烷酮、羟基化美西律和乙酸酯结构),其符合前3 d升高,后降低代谢规律,表明复方藏药可能通过调节糖代谢过程,提高机体免疫力达到治疗疾病的作用。
The components of Tibetan medicine were studied by HPLC-Q-TOF / MS with high performance liquid chromatography-time of flight mass spectrometry. Taking yak as model organism, blood sample and Tibetan medicine to BaoLingLing 1, the sample was analyzed and processed. The collected spectra were extracted by M arkerview, filtered and analyzed by principal component analysis (PCA). The results showed that there was a coexisting component in the blood of the yak of Fenghuang 1 and different dose groups. There was a significant difference in the metabolic profile between the high dose group, the low dose group, the positive drug group and the control group. The compounds with large contribution to each group were analyzed by mass spectrometry The information was matched and 80 characteristic ions were obtained. According to the database of Metlin and Massbank, eight compounds (3-ethyl-1,2-cyclopentadione, dodecenoic acid, deer grass Alkali, methyldecanoic acid, undecylenic acid, methylenedioxyflavanone, hydroxylated mexiletine and acetate structures), which was consistent with the increase in the first 3 days and then reduced the metabolic regulation, indicating that compound Tibetan medicine Probably through the regulation of glucose metabolism, improve the body’s immune system to achieve the effect of treatment of disease.