目的探讨桉叶油对维甲酸干扰大鼠胚胎植入及生长发育的影响。方法 SD孕鼠42只,随机分为6组,FA组正常对照组、溶剂组(花生油+RA)、3个实验组(桉叶油高、中、低剂量+RA)及RA组。溶剂组用花生油2ml/只于孕第7~14天灌胃,每天1次,孕第10天维甲酸40mg/kg灌胃1次。桉叶油3个剂量组分别用300mg/kg、200mg/kg、100mg/kg的桉叶油于孕第7~14天灌胃,每天1次,孕第10天维甲酸40mg/kg灌胃1次。RA组用40mg/kg的维甲酸于孕第10天灌胃1次。正常对照组给予自由摄食饮水。各组于孕21d早上处死孕鼠,取胚胎,记录孕鼠体重,子宫重,胎盘重。计胚胎植入总数,吸收胎数、活胎数、死胎数。观察胚胎外形,并测量胎鼠体重、身长、尾长。结果正常对照组、桉叶油+RA和溶剂+RA组孕鼠增重、子宫重、胎盘重与RA组比较均无差异;溶剂+RA组的孕鼠的胎盘重较其他各组低,与RA和桉叶油+RA各组比较差异无显著性,与正常对照组比较有差异(P<0.05)。正常对照组的活胎率[(97.3±4.6)%]较灌胃维甲酸各组的活胎率高(P<0.05),吸收胎率[(2.6±4.6)%]和畸形率(0)较灌胃维甲酸各组低(P<0.05),死胎率为0;在灌胃维甲酸各组中,桉叶油高[(79.6±14.5)%]、中[(67.6±30.8)%]剂量组的活胎率较RA[(58.6±26.6)%]组高,但比较无差异;畸胎率[(44.5±41.6)%;(57.5±35.1)%]较RA[(68.1±43.6)%]组低,但差异无统计学意义。灌胃维甲酸各组的吸收胎率比较无差异。正常对照组的胎鼠体重、身长、尾长均值明显高于灌胃维甲酸各组胎鼠的体重、身长、尾长均值(P<0.05);在灌胃维甲酸各组中,桉叶油各组胎鼠体重、身长的均值明显高于RA组和溶剂对照组(P<0.05);溶剂对照组胎鼠体重、身长、尾长与RA组比较无差异;桉叶油各组胎鼠尾长的均值均高于RA组,但比较无差异。结论维甲酸干扰了大鼠胚胎的植入和发育,桉叶油对RA干扰大鼠胚胎植入有一定的拮抗作用,对RA引起的胎鼠生长发育迟缓有一定的抑制作用。 Objective To investigate the effects of eucalyptus oil on retinal detachment and growth of rats induced by retinoic acid. Methods Twenty-four pregnant SD rats were randomly divided into 6 groups: normal control group, solvent group (peanut oil plus RA), three experimental groups (Eucalyptus oil high, medium and low dose + RA) and RA group. Solvent group with peanut oil 2ml / only 7th to 14th day of pregnancy gavage, day 1, the first 10 days of pregnancy retinoic acid 40mg / kg gavage 1 times. Eucalyptus oil three dose groups were 300mg / kg, 200mg / kg, 100mg / kg of eucalyptus oil in the first 7 to 14 days of pregnancy gavage once daily, the first 10 days of pregnancy retinoic acid 40mg / kg gavage 1 Times. RA group with 40mg / kg of retinoic acid intragastric administration on the 10th day of pregnancy. The normal control group was given free access to drinking water. Pregnant rats were sacrificed on the morning of pregnancy 21d, and the embryos were taken. The body weight, uterine weight and placenta weight were recorded. The total number of embryos implanted, the number of fetuses absorbed, the number of live births, the number of stillbirths. Observation of embryonic appearance, and measurement of fetal rat weight, length, tail length. Results There was no difference in weight gain, uterus weight and placental weight between normal control group, eucalyptus oil + RA + solvent + RA group and RA group. The placental weight of pregnant rats in solvent + RA group was lower than that in other groups There was no significant difference between RA and eucalyptus oil + RA groups, which was significantly different from that of normal control group (P <0.05). The percentage of live fetus in normal control group (97.3 ± 4.6%) was higher than that in each group (P0.05), and the rate of fetus fetus (2.6 ± 4.6%) and deformity rate (0% (79.6 ± 14.5)%], and [(67.6 ± 30.8)%] in the groups in which retinoic acid was given into the stomach. Compared with RA [(68.1 ± 43.6)%], the rate of live birth in the dose group was higher than that in RA [(58.6 ± 26.6)%] %] Group is low, but the difference was not statistically significant. There was no difference in the absorption rate of each group of intragastric administration of retinoic acid. The average body weight, body length and tail length of fetal rats in normal control group were significantly higher than those of the rats in each group (P <0.05). In the groups in which retinoic acid was administered, eucalyptus oil The average body weight and body length of the fetuses in each group were significantly higher than those in the RA group and the solvent control group (P <0.05). There was no difference in weight, body length and tail length of the fetal rats in the solvent control group compared with the RA group. The long mean value was higher than RA group, but no difference compared. Conclusion Retinoic acid interfered with the implantation and development of rat embryos. Eucalyptus oil could antagonize the rat embryo implantation induced by RA and inhibit the growth retardation of fetal rats induced by RA.