Ultrastructural and immunohistochemical studies on Trichomonas vagi nalis adhering to and phagocytiz

来源 :Chinese Medical Journal | 被引量 : 0次 | 上传用户:nfx0123
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Background Trichomonas vaginalis (T. vaginalis ) belongs to a common sexually transmitted disease pathogen causing genitourinary trichomoniasis in both sexes. We investigated the pathogenetic mechanism of gen itourinary trichomoniasis.Methods Cultured T. vaginalis bodies were injected into the vaginas of rats, or incubated with genitourinary epithelial cells of female subjects, male subjects, and sperm. The ultrastructural and microscopic changes were observed via transmission and scanning electron microscopy and through microscopic histochemistry.Results Groups of T.vaginalis adhered to PAS positive colum nar cells at the surface of stratified epithelium in the middle and upper portions of the vaginas. They a lso traversed under these cells. The parasites were shown to be PAS, cathepsin D, and actin positive, and they could release hydrolase into the cytoplasm of adher ed epithelial cells. In the amebiform T.vaginalis, microfilaments were arranged into reticular formation. Similar phenomena were found during the interaction of T.vaginalis with host cells, both in vitro and in vivo. Usually several protozoa adhered to a n epithelial cell and formed polymorphic pseudopodia or surface invaginations to surround and phagocytize the microvilli or other parts of the epithelial cytoplasm. Adhesion and phagocytosis of sperm by the protozoa occurred at 15-30 minutes of incubation. Digestion of sperm was found at 45-75 minutes and was complete at 90-105 minutes.Conclusions T.vaginalis tends to parasitize at the fornix o f the vagina, because this is the site where columnar cells are rich in mucinogen granules and thei r microvilli are helpful for adhesion and nibbling. T.vaginalis possesses some invad ing and attacking abilities. Shape change, canalization, encystation, phagocytosis, digestion, the cell coat, cytoskeleton, and lysosome all play important roles in the process of adhesion. They have two methods of phagocytosis: nibbling and ingestion. Genitourinary epithelium may be injured directly by the digestive action of hydrolases, phagocytosis, and the mechanical action of pseudopodia. Background Trichomonas vaginalis (T. vaginalis) belongs to a common sexually transmitted disease pathogen causing genitourinary trichomoniasis in both sexes. We investigated the pathogenetic mechanism of gen it it canine trichomoniasis. Methods Cultured T. vaginalis bodies were injected into the vaginas of rats, or incubated with gen ultrasurgial epithelial cells of female subjects, male subjects, and sperm. The ultrastructural and microscopic changes were observed via transmission and scanning electron microscopy and through microscopic histochemistry. Results Groups of T. vaginalis adhered to PAS positive colum nar cells at the surface of stratified epithelium in the middle and upper portions of the vaginas. They a lso traversed under these cells. The parasites were shown to PAS, cathepsin D, and actin positive, and they could release hydrolase into the cytoplasm of adher ed epithelial cells. In the amebiform T.vaginalis, microfilaments were arranged into reticular formation. Similar ph enomena were found during the interaction of T. vasinalis with host cells, both in vitro and in vivo. Both several protozoa adhered to an epithelial cell and formed polymorphic pseudopodia or surface invaginations to surround and phagocytize the microvilli or other parts of the epithelial cytoplasm. Adhesion and phagocytosis of sperm by the protozoa occurred at 15-30 minutes of incubation. Digestion of sperm was found at 45-75 minutes and was complete at 90-105 minutes. Conlusions T. vasinalis tends to parasitize at the fornix of the vagina, because this is the site where columnar cells are rich in mucinogen granules and thei r microvilli are helpful for adhesion and nibbling. T. vaginalis possesses some invad ing and attacking abilities. Shape change, canalization, encystation, phagocytosis, digestion, the cell coat, cytoskeleton, and lysosome all play important roles in the process of adhesion. They have two methods of phagocytosis: nibbling and ingestion. Genitourinary epitheliummay be injured directly by the digestive action of hydrolases, phagocytosis, and the mechanical action of pseudopodia.
其他文献
一、我国社会保障制度现状及其弊端 我国现行的养老、失业等社会保障制度是从1986年开始实行的。在此之前,我国一直实行的是“企业保障”。现行的社会保障制度主要是现收现
主题一:积极财政政策不能长期化 财政政策支持经济与自身的可持续发展都要考虑,如果不考虑财政的承受能力或考虑得不够,不断地把赤字扩大下去,把积极财政政策搞下去,我认为
一、政府间税种配置存在的理论分析 为什么要在各级政府间进行税种配置呢?对这一问题,施蒂格勒、蒂博特、布坎南、沃茨及特里西等西方经济学者作了最深刻的阐述。他们首先依
大鼠和家犬单次及多次给予卡铂均可引起骨髓造血功能严重抑制、肠粘膜等多个脏器出血.淋巴组织萎缩,肝细胞和肠粘模上皮细胞变性、坏死、生精抑制等。上述毒性表现与剂量相关
氨铂(Am-DACHP),化学名为氨基丙二酸-1,2-二氨基环已烷合铂(Ⅱ)(1),是顺铂的衍生物,属第二代铂配合物抗癌药。氨铂化学稳定性好,水溶性大,动物实验表明,其抗 L 1210白血病和
随着我国教育体制的改革进程的推进,对高校的美术课程教学的要求越来越高。目前,高等学校美术课程的开设目的不但是提高大学生的绘画技能,而且塑造大学生优秀的创新精神与艺
所谓策展人,顾名思义即(艺术)展览中负责构思、策划、管理等的专职人员。在西方文化中,策展人的角色是在艺术宫、展览馆等研究、保管或策划、管理展览的专职人员。从历史发展
从全疆不同植棉区采集棉花枯萎病病株,经分离纯化和单孢分离获得尖孢镰刀菌菌株,从中选20个代表菌株,用目前国内外统一使用的一套鉴别寄主进行生理小种鉴定,初步鉴定结果表明:新疆棉
“也许这一切都是乌托邦式的梦。但正是这些革命运动带来的梦,除了某些短暂的时刻,也许无法真正实现他们的梦,但却让嬉皮士以及他们的前赴后继者在他们的‘时刻’开始有了想
对HIV-1逆转录酶的抑制是目前被认为是预防爱滋病有益的新进展。天然产物已广泛用作HIV-1逆转录酶(HIV-1RT)的抑制剂。作者开发的在病毒粒子中检测HIV-1的方法,可作为天然产