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用体内及体外两种实验方法 ,研究了谷氨酸对鼠脑组织中过氧化脂质及还原型谷胱甘肽含量的影响 ,以及施用褪黑素 (MT)后对谷氨酸的对抗作用 .结果表明 ,在体外 ,不同浓度的谷氨酸(2 9～ 11 7mmol/L )均能显著提高大鼠脑匀浆中丙二醛 (MDA )的含量 ;而加入MT(1～ 10mmol/L) ,可显著的对抗这种升高 .体内实验 ,小鼠侧脑室给予不同浓度的谷氨酸可显著升高全脑组织中丙二醛 (MDA)的含量及降低还原型谷胱甘肽的含量 ;如预先腹腔注射MT(1～ 10mg·kg-1) ,可显著对抗谷氨酸的影响 .MT的这种作用可能是其抗衰老的机制之一 .
The effects of glutamate on lipid peroxidation and glutathione content in brain tissue of mice and the antagonism of glutamate after applying melatonin (MT) were studied in vivo and in vitro The results showed that in vitro, different concentration of glutamic acid (29 ~ 11 7mmol / L) can significantly increase the content of malondialdehyde (MDA) in rat brain homogenate; while adding MT (1 ~ 10mmol / L ), Can significantly antagonize this increase.In vivo experiments, different concentrations of glutamate in mice lateral ventricle can significantly increase the content of malondialdehyde (MDA) in whole brain tissue and reduce the reduction of glutathione (P <0.05) .It could significantly antagonize the effect of glutamic acid (MT) by intraperitoneal injection of MT (1 ~ 10 mg · kg-1) .The effect of MT may be one of its anti-aging mechanisms.