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目的采用RP-HPLC法进行兰索拉唑的血药浓度检测和药动学研究。方法采用Agilent C18色谱柱(250 mm×5mm,5μm),流动相为乙腈-1‰三乙胺水溶液(pH7)(30∶70),流速1.0 mL.min-1,进样量20μL,内标为奥美拉唑。血浆样品经乙酸乙酯提取后,于285 nm处检测。结果 0.05～1.60μg·mL-1兰索拉唑与峰面积比值的线性关系良好(r=0.9997),最低定量限为20 ng.mL-1(S/N>3),日内RSD<7.94%(n=5),日间RSD<9.41%(n=5),提取回收率>95.79%。结论所用方法可用于临床上兰索拉唑片血药浓度的检测及药动学研究。
Objective To study the plasma concentration of lansoprazole and its pharmacokinetics by RP-HPLC. Methods The Agilent C18 column (250 mm × 5 mm, 5 μm) was used. The mobile phase was acetonitrile-1 ‰ triethylamine (pH7) (30:70) and the flow rate was 1.0 mL.min-1. Omeprazole. Plasma samples were extracted with ethyl acetate and detected at 285 nm. Results The linear relationship between lansoprazole and peak area ratio was good (r = 0.9997), the lowest limit of quantification was 20 ng.mL-1 (S / N> 3), the intra-day RSD was less than 7.94% (n = 5), daytime RSD <9.41% (n = 5), extraction recovery> 95.79%. Conclusion The method can be used for the clinical detection of lansoprazole tablets and pharmacokinetic studies.