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为了研究国内主要商业蓖麻品种的种质资源多样性,采用AP-PCR与RMAPD分子标记方法,对来自国内不同地区的31份蓖麻品种进行分析。AP-PCR分子标记的研究结果表明,9条引物扩增的条带为5~12条,共计82条带,平均每条引物扩增出9.1条带,扩增的多态性条带为2~10条,多态率为40%~90.91%,共计53条带,平均每条引物扩增出5.89条多态性带。在9条引物中,引物S3扩增出多态性条带最高,多态率达90.91%。蓖麻种质资源的RMAPD分析结果表明,84对引物扩增的条带为5~12条,共计85条带,平均每对引物扩增出8.5条带,扩增的多态性条带为3~10条,共计56条带,平均每条引物扩增出5.6条多态性带。在84对引物中,引物A3+B4扩增出的条带最多,多态性最高,多态率达83.3%。对AP-PCR与RMAPD分子标记的数据进行联合聚类分析表明,在遗传系数为0.56处,31份蓖麻种质被分成3大类群。2种分子标记之间的相关系数不显著(R=0.009 3)。
In order to study the diversity of germplasm resources of major commercial castor varieties in China, 31 castor varieties from different regions of China were analyzed by AP-PCR and RMAPD molecular markers. The results of AP-PCR molecular marker showed that the amplified bands of 9 primers were 5 to 12, with a total of 82 bands, with an average of 9.1 bands per primer, and the amplified polymorphic bands were 2 ~ 10, the polymorphic rate was 40% ~ 90.91%, a total of 53 bands, with an average of 5.89 per primer amplified polymorphic bands. Among the 9 primers, primer S3 amplified the highest polymorphic bands with a polymorphic rate of 90.91%. The result of RMAPD analysis of castor germplasm resources showed that 84 pairs of primers amplified 5 to 12 bands, a total of 85 bands, with an average of 8.5 bands amplified for each pair of primers. The amplified polymorphic bands were 3 to 10, a total of 56 bands, an average of each primer amplification of 5.6 polymorphic bands. Among the 84 pairs of primers, the amplified bands of the primers A3 + B4 were the most, with the highest polymorphism rate of 83.3%. Cluster analysis of AP-PCR and RMAPD molecular marker data showed that at the genetic coefficient of 0.56, 31 castor germplasms were divided into three groups. The correlation coefficient between the two kinds of molecular markers was not significant (R = 0.009 3).