论文部分内容阅读
目的:观察中药复方糖耐康对肥胖Zucker大鼠糖代谢和胰岛素抵抗的影响。方法:6周龄雄性肥胖Zucker大鼠12只,适应性喂养2周后,随机分为对照组和糖耐康组(3 .24 g/kg) ,所有大鼠给予高脂饲料喂养,疗程为4周。每周检测体质量和血糖;入组前和治疗14、28 d时行口服葡萄糖耐量实验(oral glucose tolerance test ,OGTT)并检测空腹血清胰岛素水平;第28天时检测空腹血脂4项和血浆游离脂肪酸(free fatty acids ,FFA)水平;第29天时进行高胰岛素正葡萄糖钳夹实验检测平均葡萄糖输注率(glucose infusion rate ,GIR) ;实验结束后处死大鼠并取材,检测骨骼肌中蛋白激酶B(protein kinase B,PKB/Akt)、磷酸化蛋白激酶B(phospho-Akt , p-Akt/Thr308)、葡萄糖转运蛋白4(glucose transporterprotein 4 ,GLUT4)的表达和脂肪组织GLUT4的蛋白表达。结果:与对照组相比,治疗4周后,糖耐康组血清胰岛素水平没有变化,餐后血糖和OGTT中120 min时的血糖水平均显著下降;糖耐康组高胰岛素正葡萄糖钳夹实验后GIR显著提高;糖耐康能显著增加骨骼肌Akt、p-Akt(Thr308)的蛋白表达,减少脂肪组织GLUT4的蛋白表达;糖耐康有降低体质量、血脂(三酰甘油、胆固醇、低密度脂蛋白)和FFA含量的趋势,但两组比较差异无统计学意义。结论:糖耐康可能是通过增加骨骼肌Akt和p-Akt(Thr308)的表达,增强GLUT4的葡萄糖转运能力来实现降低血糖,改善外周胰岛素抵抗的功效。
Objective: To observe the effect of Tangjikang, a traditional Chinese medicine, on glucose metabolism and insulin resistance in obese Zucker rats. Methods: Twelve male Zucker rats, 6 weeks old, were randomly divided into control group and candyongkang group (3.24 g / kg) after 2 weeks of adaptive feeding. All rats were given high fat diet for a period of 4 weeks. Body mass and blood glucose were measured weekly. Oral glucose tolerance test (OGTT) and fasting serum insulin levels were measured before treatment and 14, 28 d after treatment. On the 28th day, four fasting blood lipids and plasma free fatty acids (FFA). On the 29th day, the hyperinsulinemic positive glucose clamp test was performed to detect the average glucose infusion rate (GIR). At the end of the experiment, the rats were sacrificed and the contents of protein kinase B The expression of protein kinase B (PKB / Akt), phospho-Akt (p-Akt / Thr308), glucose transporter protein 4 (GLUT4) and the expression of GLUT4 in adipose tissue were detected. Results: Compared with the control group, after 4 weeks of treatment, there was no change in the serum insulin level in the glucose-resistant group and the postprandial glucose level and the blood glucose level in the OGTT at 120 min were significantly decreased. Glucose-resistant hyperinsulinemic-glucose clamp test Glucose significantly increased skeletal muscle Akt, p-Akt (Thr308) protein expression, reduce adipose tissue GLUT4 protein expression; Tang Kang Kang have to reduce body weight, blood lipids (triglyceride, cholesterol, low Density lipoprotein) and FFA content trends, but there was no significant difference between the two groups. CONCLUSION: IGT may reduce the blood glucose and improve peripheral insulin resistance by increasing the expression of Akt and p-Akt (Thr308) in skeletal muscle and enhancing the glucose transport ability of GLUT4.