论文部分内容阅读
以木立芦荟的叶片、叶鞘、带腋芽的茎段为外植体进行试管培养 ,结果叶鞘和茎段可诱导形成愈伤组织 ,腋芽直接萌生。经试验筛选出各培养阶段最适宜的培养基为 :( 1 )愈伤组织诱导 ,MS +BA 2 .5mg/L +NAA0 .1 5mg/L ;( 2 )腋芽萌生 ,MS +BA 2 .0mg/L +NAA 0 .1 5mg/L ;( 3 )丛生芽分化及继代 ,MS +BA 2 .0mg/L +NAA0 .1 0mg/L ;( 4)生根 ,MS +BA 0 .3～ 0 .5mg/L +IBA 0 .2mg/L +活性碳 0 .5%。研究还发现 ,培养基酸碱度对木立芦荟组织培养分化效果的影响非常显著。
Aloe arborescent leaves, leaf sheaths, axillary buds with stem segments as explants for tube culture, the results of leaf sheath and stem segments can be induced to form callus, axillary buds directly initiation. The most appropriate medium for each culture stage was screened out as follows: (1) callus induction, MS + BA 2 .5mg / L + NAA0.1 5mg / L; (2) axillary bud initiation, MS + BA 2 .0mg / L + NAA 0 .1 5mg / L; (3) Cluster shoot differentiation and subculture, MS + BA 2 .0mg / L + NAA0.100mg / L; .5mg / L + IBA 0 .2mg / L + activated carbon 0.5%. The study also found that the pH value of culture medium Aloe arborescens tissue culture differentiation effect is very significant.