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以辣椒材料‘P205’和‘野力姆’为砧木,以‘三道筋’为接穗进行嫁接,研究了嫁接对高温(45±1)℃和低温(4±1)℃胁迫下辣椒快速叶绿素荧光诱导动力学特性的影响。结果表明:温度胁迫引起自根苗叶片快速叶绿素荧光诱导曲线(OJIP)发生显著变化,J、I、P相降低明显。JIP-test分析显示,荧光诱导曲线中出现了明显K相,且自根苗和嫁接苗在高温胁迫后的ΔK值均高于低温胁迫,说明辣椒叶片光合机构更易受到高温胁迫的损伤。温度胁迫降低了自根苗和嫁接苗的最大光化学效率(F_v/F_m)、性能指数(PI_(abs)),其中PI_(abs)对温度胁迫更敏感。自根苗和嫁接苗叶片的反应中心单位面积上吸收(ABS/CS_m)、捕获(TR_o/CS_m)和传递(ETo/CS_m)的光能以及反应中心数量(RC/CS_m)在温度逆境中下降,而J相相对可变荧光(VJ)、热耗散量子比率(Ψ_(Do))、单位面积的热耗散(DI_o/CS_m)和综合性能指数(PItotal)升高,其中自根苗所有荧光参数值均与常温对照差异显著。砧木‘P205’嫁接苗和砧木‘野力姆’嫁接苗分别对高温和低温胁迫具有较好的适应性。自根苗和嫁接苗叶片的PI_(total)在温度逆境下均大幅升高,胁迫解除后又迅速恢复,表明在温度胁迫下辣椒叶片光系统Ⅰ(PSⅠ)的受损伤程度较PSⅡ低。
Using pepper materials ’P205’ and ’wild rice’ as rootstocks and grafting ’three tendons’ as scions, the effects of grafting on the chlorophyll fluorescence of pepper under high temperature (45 ± 1) ℃ and low temperature (4 ± 1) ℃ Effect of induced kinetic properties. The results showed that temperature stress caused a significant change in the rapid chlorophyll fluorescence induction curve (OJIP) of self-rooted shoots, while the J, I and P phases decreased significantly. JIP-test analysis showed significant K phase in the fluorescence induction curve, and the ΔK values of self-rooted and grafted seedlings were higher than those under low temperature stress after high temperature stress, indicating that the photosynthetic mechanism of pepper leaves is more vulnerable to high temperature stress. Temperature stress reduced the maximum photochemical efficiency (F_v / F_m) and PI_ (abs) of self-rooted and grafted seedlings, of which PI_ (abs) was more sensitive to temperature stress. (ABS / CS_m), light energy (ET / CS_m) and the number of reaction centers (RC / CS_m) of trapping seedling and grafted seedling leaves decreased in temperature stress, While J phase relative variable fluorescence (VJ), heat dissipation quantum ratio (Ψ_ (Do)), heat dissipation per unit area (DI_o / CS_m) and PItotal increased. Among all the fluorescence parameters Values were significantly different from the normal temperature control. The rootstock ’P205’ grafted seedlings and the rootstock ’Wild Rose’ grafted seedlings had good adaptability to high temperature and low temperature stress, respectively. The PI (total) of self-rooted and grafted seedlings increased significantly under the temperature stress and recovered rapidly after the stress was relieved, indicating that the damage of PSⅠ in pepper leaves was lower than that in PSⅡ under temperature stress.