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目的建立中国健康成人外周血B淋巴细胞各种免疫表型标志的参考范围,为临床正确诊断B淋巴细胞增殖性疾病提供基础信息。方法采用全血溶解法和SSC/CD19设门技术以三色流式细胞术对来自41例健康在成人EDTA-K2抗凝静脉血进行免疫表型分析。结果外周血B淋巴细胞几乎全部表达CD22、CD20、CD62L、CD24、CD79b、CD79a和FMC-7分子,几乎不表达CD11a、CD80、CD103、CD10、CD40L、CD54、CD95L、CD86和CD95分子,CD18、CD44、CD23、CD5、CD11c和CD43的阳性率各不相同,78%的B细胞为IgD阳性、κ/γ比例为1.26。结论为了对患者的免疫分型资料进行准确判读和对B淋巴细胞增殖性疾病作出正确的诊断,必须考虑正常B细胞群体中各种标志的表达情况。中国人和西方人群在某些B细胞标志方面存在的差异,临床工作者不宜直接套用国外的标准。
Objective To establish a reference range of various immunophenotypic markers of peripheral blood B lymphocytes in healthy Chinese adults and provide basic information for the correct diagnosis of B lymphoproliferative diseases. Methods The immunophenotypes of 41 healthy adults with anticoagulated EDTA-K2 venous blood were analyzed by the three-color flow cytometry using whole blood lysate and SSC / CD19 portal approach. Results CD22, CD20, CD62L, CD24, CD79b, CD79a and FMC-7 molecules were expressed almost exclusively in peripheral blood B lymphocytes. CD11a, CD80, CD103, CD10, CD40L, CD54, CD95L, The positive rates of CD44, CD23, CD5, CD11c, and CD43 vary, with 78% of B cells being IgD positive with a κ / γ ratio of 1.26. Conclusion In order to accurately interpret the patient’s immunophenotyping data and correctly diagnose B lymphoproliferative diseases, we must consider the expression of various markers in normal B cell population. Chinese and Western populations in some B cell markers exist differences, clinicians should not directly apply foreign standards.